B4B Earth Tea Immune Support Drink: Study Shows Real Results, Defying Misconceptions and Slander
Objective:
The purpose of this study was to assess the ability of different concentrations of a sample (Earth Tea) to inhibit the binding of the COVID-19 spike protein to its human receptor, ACE2, under two conditions (unfiltered and filtered samples, at both 4°C and room temperature). Binding inhibition was evaluated by measuring optical density (OD) values and calculating the percentage of inhibition in comparison to a ligand-only control.
Methodology:
1. Sample Preparation and Experimental Setup:
• Different concentrations of the Earth Tea sample were prepared: 99%, 24.75%, 6.188%, 1.547%, 0.387%, and 0.097% (v/v).
• The samples were incubated at two different temperatures: 4°C and room temperature (RT).
• Two sets of samples were used: unfiltered and filtered using Amicon® Ultra Centrifugal Filters.
2. Controls:
• SACE2-Controls-PC (positive control) and SACE2-Controls-NC (negative control) were used to validate the assay’s performance.
• The Ligand Only Control provided a reference for the baseline binding activity, while the ADB Only Blank was used for background correction.
3. Binding Inhibition Calculation:
• The optical density (OD) of each sample was measured in triplicate (OD1, OD2, and OD3) to ensure accuracy and consistency.
• The Background Corrected Average OD was calculated by subtracting the ADB Only Blank OD from the sample’s average OD.
• Binding inhibition was calculated using the following formula:
• Results were recorded for each concentration at both temperatures.
Results:
The data for the Unfiltered Sample at 4°C (99% concentration) is presented as follows:
Sample Concentration (v/v) OD1 OD2 OD3 Average CV% BG Corrected AVG Average Binding Inhibition
Unfiltered Sample 99% 0.104 0.086 0.085 0.09167 11.7% 0.04400 98.398%
• Background Correction: The background-corrected average OD of the unfiltered sample at 99% concentration was calculated as 0.04400.
• Binding Inhibition: The sample achieved 98.398% inhibition, which indicates that the sample is highly effective at preventing the COVID-19 spike protein from binding to its receptor.
Additional results for other concentrations and conditions, including the filtered samples and room temperature conditions, were also measured. These results are summarized below:
Key Findings for Binding Inhibition (Average Inhibition):
1. Unfiltered Samples (4°C):
• 99% concentration: 98.398% inhibition
• 24.75% concentration: 44.872% inhibition
• 6.188% concentration: -1.626% inhibition (indicating no significant inhibition)
• 1.547% concentration: 9.528% inhibition
• 0.387% concentration: -8.909% inhibition (indicating a negative result)
2. Filtered Samples (4°C):
• 99% concentration: 74.536% inhibition
• 24.75% concentration: 18.364% inhibition
• 6.188% concentration: 17.514% inhibition
• 1.547% concentration: 12.125% inhibition
• 0.387% concentration: 7.125% inhibition
3. Unfiltered Samples (Room Temperature):
• 99% concentration: 98.750% inhibition
• 24.75% concentration: 51.438% inhibition
• 6.188% concentration: -0.837% inhibition
• 1.547% concentration: 17.539% inhibition
• 0.387% concentration: 5.425% inhibition
4. Filtered Samples (Room Temperature):
• 99% concentration: 83.930% inhibition
• 24.75% concentration: 10.123% inhibition
• 6.188% concentration: 6.081% inhibition
• 1.547% concentration: 12.465% inhibition
• 0.387% concentration: 4.649% inhibition
Discussion:
1. Effectiveness at Higher Concentrations:
• The highest concentration of 99% (v/v) Earth Tea consistently showed the highest inhibition percentages, ranging from 98.398% (unfiltered, 4°C) to 98.750% (unfiltered, room temperature), indicating that higher concentrations of the sample are more effective at inhibiting the binding of the COVID-19 spike protein.
• The filtered samples generally showed lower binding inhibition compared to the unfiltered samples, possibly due to the loss of active components during the filtration process.
2. Temperature Effects:
• The room temperature conditions generally resulted in higher binding inhibition percentages compared to the 4°C condition, particularly at the 99% concentration, suggesting that temperature may enhance the sample’s effectiveness in inhibiting the binding.
3. Low and Negative Inhibition:
• At lower concentrations (e.g., 6.188% and 0.387%), the binding inhibition was much lower, with some negative results (e.g., -1.626% at 6.188% concentration, unfiltered). This indicates that below a certain concentration, Earth Tea may not be effective in inhibiting the binding of the COVID-19 spike protein.
4. Controls:
• The positive control (SACE2-Controls-PC) achieved 80.714% inhibition, confirming that the assay is working as expected.
• The negative control (SACE2-Controls-NC) showed 5.753% inhibition, indicating minimal inhibition, which aligns with expectations for a negative control.
Conclusion:
Based on the results of this binding inhibition assay, the 99% (v/v) Earth Tea sample demonstrated excellent inhibitory activity, with 98.398% inhibition at 4°C and 98.750% inhibition at room temperature, making it a promising candidate for further investigation as a potential inhibitor of the COVID-19 spike protein binding. Higher concentrations (99%) are significantly more effective compared to lower concentrations, and temperature appears to enhance the effectiveness of Earth Tea. However, the effectiveness is significantly reduced at lower concentrations, suggesting a threshold concentration below which Earth Tea may not provide significant inhibitory activity.
Scientists here’s the ELISA Report Attached.
